Studies on Kinetics of Alkaline Protease Production of by Bacillus licheniformis


  • Dr Muhammad Sagir University of gujrat


Proteases are the most important enzymes produced by Bacillus licheniformis. The protease producer strain Bacillus licheniformis GCU-12 was isolated from the soil. Fermentation medium containing Glucose 5.0; Peptone 7.5; NaCl 5.0; MgSO4.7H2O 5.0; FeSO4.7 H2O 0.1; was used for the production. The production was investigated in stirred fermenter as well as shake flasks. In stirred fermenter the culture produced 56 U/cm3during 24 h fermentation At 9.0 and 40oC. The kinetic and mass balance studies revealed that the growth rate was 0.25 h-1 during first 12 hrs of fermentation in the stirred fermenter and 0.183 h-1 for first 18 hrs of fermentation in shake flasks. The value of number of generations and its doubling time in the fermenter were 4.33 and 2.8 h whereas the number of generations and the doubling time for shake flasks were 4.8 and 3.79 h. The substrate uptake rate of biomass was 2.9 g/dm3/hour in fermenter and 1.8 g/dm3/hour for shake flasks while specific substrate uptake rate was maintained 0.2 g substrate-g-1 biomass-h-1 during exponential phases both in the shake flask and the stirred fermenter.



How to Cite

Sagir, D. M. (2017). Studies on Kinetics of Alkaline Protease Production of by Bacillus licheniformis. Journal of the Pakistan Institute of Chemical Engineers, 45(2). Retrieved from