Comprehensive chemical profiling and biological activities of Tinospora malabarica: Implications for herbal drug development and quality control

Authors

  • Khadim Ali Gishkori Pakistan Standard Quality Control Authority, Karachi Pakistan
  • Saba Naz Dr. M. A. Kazi Institute of Chemistry, University of Sindh, 76080, Jamshoro Pakistan
  • Aftab Ahmed Kandhro Dr. M. A. Kazi Institute of Chemistry, University of Sindh, 76080, Jamshoro-Pakistan

DOI:

https://doi.org/10.54693/piche.05411

Abstract

This study investigates the chemical composition and biological activities of a significant medicinal plant species, Tinospora malabarica, Sindh region of Pakistan. Initially, the determination of physicochemical parameters was carried out to understand the general properties of plant. Phenolic compounds were identified using HPLC-PDA and 6 phenolics were detected in this species. Essential oils and volatile compounds were extracted through steam distillation. Analysis of each volatile constituent was done through GC-MS and 75 compounds were identified. Fatty acid profiling was conducted via GC-FID, and identified 19 fatty acids in T. malabarica. To evaluate the antioxidant capacity, BHT was used as control in DPPH assay. Antibacterial activity was evaluated by testing the extracts against five bacterial strains: Proteus mirabilis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhimurium, Staphylococcus aureus. The results provide a comprehensive understanding of this plant species' chemical composition and biological activities, highlighting its potential for the development of herbal drug formulations. Furthermore, the findings contribute to establishing quality control protocols for use of T. malabarica which is a raw materials used in herbal drug production.

Published

2026-04-23

How to Cite

Gishkori, K. A., Naz, S., & Kandhro, A. A. (2026). Comprehensive chemical profiling and biological activities of Tinospora malabarica: Implications for herbal drug development and quality control. Journal of the Pakistan Institute of Chemical Engineers, 54(1). https://doi.org/10.54693/piche.05411

Issue

Vol. 54 No. 1 (2026): Journal of the Pakistan Institute of Chemical Engineers

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).